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silica extraction phenol

Use of silica gel polymer for DNA extraction with

1989-11-15  With the use of silica gel polymer containing tubes, phenol, phenol:chloroform, or chloroform can be separated from the DNA containing aqueous phase in a rapid and safe manner. The method permits higher yields of DNA since the DNA is poured from the tube rather than aspirated with pipet. Ki 1989 Academic Press, Inc.

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Highly effective DNA extraction method for nuclear

Aim: To quantitatively compare a silica extraction method with a commonly used phenol/chloroform extraction method for DNA analysis of specimens exhumed from mass graves. Methods: DNA was extracted from twenty randomly chosen femur samples, using the International Commission on Missing Persons (ICMP) silica method, based on Qiagen Blood Maxi Kit, ...

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Use Solid Phase Extraction to Isolate Phenols from

2020-11-14  Solid phase extraction (SPE) methods have been developed for many hydrophobic analytes, but extractions of hydrophilic phenols are difficult because these compounds have a strong tendency to hydrogen bond with water molecules. Extraction of phenols using silica-based SPE packings has yielded both poor

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A study on the oxidation of phenol by heterogeneous

2010-12-1  A series of iron silica catalyst with (5–20) wt.% Fe 3+ were prepared by means of a simple solvent extraction and sol–gel technique. These catalysts were probed for the oxidation of phenol employing hydrogen peroxide. Catalytic performance increased up to 10 wt.% Fe 3+ loading. Further increase in the iron content was found to reduce the phenol conversion rate.

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Solid‐phase silica‐based extraction leads to ...

2020-9-15  This method is often preferred over the phenol/chloroform-based extraction, as is does not rely on hazardous chemicals. 8, 10 Solid-phase extraction exploits interactions of DNA with a solid substrate, such as silica resin/beads in the presence of chaotropic salts, allowing for rapid purification of DNA

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SELECTIVE SEPARATION OF PHENOLIC COMPOUND

2015-3-3  1.1 Molecular structure of phenol. 3 2.1 Molecular Imprinting Technique (MIP) 13 2.2 Sep -Pak Vac 35cc. (silica C 18, 10g) 17 2.3 3.1 Solid Phase Extraction Steps Flow of Methodology 21 26 3.2 Molecular imprinting polymer technique 28 3.3 Solid Phase Extraction 30 4.1 Effect of

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Improve sample preparation process for miRNA

2020-12-3  Xu, L. et al. Altered nucleic acid partitioning during phenol extraction or silica adsorption by guanidinium and potassium salts. Anal. Biochem. 419, 309–316.

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Silica Bead DNA Gel Extraction Kit - Thermo Fisher

Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water. This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods.

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'Method 528 - Determination of Phenols in Drinking

2015-9-17  4.4 Airborne phenol may be a source of phenol contamination in samples and sample extracts. Samples should not be stored or extracted in areas where phenol is used for other laboratory operations. 4.5 2,3,4,5-Tetrachlorophenol is used as one of the internal standards for the quantitation of reactive and thermally labile phenols.

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DNA EXTRACTION METHODS

2020-6-14  • Phenol/Chlorform - The standard way to remove proteins from nucleic acids solutions is to extract once with phenol, once with a 1:1 mixture of phenol and chloroform, and once with chloroform. This procedure takes advantage of the fact that deproteinization is more efficient when two different organic solvents are used instead of one.

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(PDF) Paper prove Silica method better than Phenol ...

Forensic Science Highly Effective DNA Extraction Method for Nuclear Short Tandem Repeat Testing of Skeletal Remains from Mass Graves Jon Davoren1,2, Daniel Vanek1,3, Rijad Konjhodzić1,4, John Crews1,2, Edwin Huffine1,2, Thomas J. Parsons1 1 The International Commission Aim To quantitatively compare a silica extraction method with a on Missing Persons, Sarajevo, commonly used phenol

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Highly effective DNA extraction method for nuclear

Aim: To quantitatively compare a silica extraction method with a commonly used phenol/chloroform extraction method for DNA analysis of specimens exhumed from mass graves. Methods: DNA was extracted from twenty randomly chosen femur samples, using the International Commission on Missing Persons (ICMP) silica method, based on Qiagen Blood Maxi Kit, and compared with the DNA

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Use of silica gel for DNA extraction with organic

Enzymol 152: 180-183 (1987), involves differentiated solvation of the DNA and the non-DNA material, using phenol and chloroform. In this standard methodology, following a series of preparative steps the DNA containing sample is mixed with an organic solvent, such as phenol, or preferably, an equal mixture of phenol and chloroform.

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DNA Extraction and Polymerase Chain Reaction

2021-6-18  DNA extraction techniques include organic extraction (phenol–chloroform method), nonorganic method (salting out and proteinase K treatment), and adsorption method (silica–gel membrane). Organic extraction. This method is labor intensive and time consuming.

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View of A cost-effective RNA extraction technique

RNA is generally extracted by two methods: phenol-chloroform or commercially available silica spin column kits. Phenol-chloroform extraction is generally more economical; however, it produces hazardous byproducts, and leftover chemicals in the sample that can inhibit downstream applications.

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Adsorption of phenol over bio-based silica/calcium ...

2020-10-13  A bio-based Silica/Calcium Carbonate (CS–SiO2/CaCO3) nanocomposite was synthesized in this study using waste eggshells (ES) and rice husks (RH). The adsorbents (ESCaCO3, RHSiO2 and, CS-SiO2/CaCO3) characterized using XRD show crystallinity associated with the calcite and quartz phase. The FTIR of ESCaCO3 shows the CO−23 group of CaCO3, while the spectra of RHSiO2

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Silica Bead DNA Gel Extraction Kit - Thermo Fisher

Thermo Scientific Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie, employing solubilization of the agarose gel and selective adsorption of nucleic acids

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DNA purification and isolation of genomic DNA from ...

2014-6-23  5) Organic extraction using phenol (Wang and Rossman, 1994). Promega products like the Wizard® Plus SV Minipreps DNA Purification System and the PureYield™ Plasmid Systems combine the benefits of alkaline lysis with the rapid and easy purification by silica. This is done by using a silica-based membrane in a column format to bind the

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DNA isolation methods - University of Alberta

2012-1-8  Organic extraction is a conventional technique that uses organic solvents to extract contaminants from cell lysates (3, 4). The cells are lysed using a detergent, and then mixed with phenol, chloroform, and isoamyl alcohol. The correct salt concentration and pH must be used during extraction to ensure that contaminants are separated into

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[Study on analysis of trace phenol in small amount of ...

[Study on analysis of trace phenol in small amount of aqueous sample by liquid-liquid micro-extraction and gas chromatography]. [Article in Chinese] Zhang AL(1), Zhou JT, Han M, Teng LM, Huang LP, Wang D, Wang J. Author information: (1)School of Environmental Science and Engineering, Dalian University of Technology, Dalian 116012, China.

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(PDF) Paper prove Silica method better than Phenol ...

Forensic Science Highly Effective DNA Extraction Method for Nuclear Short Tandem Repeat Testing of Skeletal Remains from Mass Graves Jon Davoren1,2, Daniel Vanek1,3, Rijad Konjhodzić1,4, John Crews1,2, Edwin Huffine1,2, Thomas J. Parsons1 1 The International Commission Aim To quantitatively compare a silica extraction method with a on Missing Persons, Sarajevo, commonly used phenol

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Photocatalytic degradation of phenol using silica SBA

2020-6-29  Silica SBA-16, an efficient photocatalyst support with cubic (bottle-ink) mesoporous structure, large specific surface area, large pore volume and uniform pore size and titanium oxide (TiO2), a semiconductor compound as the active site were synthesized facilely via one-pot method and photocatalysts denoted as x%TiO2/SBA-16 where x = 2, 5, 10 and 20, in order to examine their

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A Sample Extraction Method for Faster, More

2012-1-13  Three mechanistically different sample extraction methodologies, namely, silica spin columns, phenol-chloroform, and an automated magnetic capture of polymer-complexed DNA (via an Automate Express instrument), were compared for their abilities to purify nucleic acids from blood culture fluids for use in TaqMan assays for detection of Staphylococcus aureus. The extracts from silica

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Adsorption of phenol over bio-based silica/calcium ...

2020-10-13  A bio-based Silica/Calcium Carbonate (CS–SiO2/CaCO3) nanocomposite was synthesized in this study using waste eggshells (ES) and rice husks (RH). The adsorbents (ESCaCO3, RHSiO2 and, CS-SiO2/CaCO3) characterized using XRD show crystallinity associated with the calcite and quartz phase. The FTIR of ESCaCO3 shows the CO−23 group of CaCO3, while the spectra of RHSiO2

More

SELECTIVE SEPARATION OF PHENOLIC COMPOUND

2015-3-3  1.1 Molecular structure of phenol. 3 2.1 Molecular Imprinting Technique (MIP) 13 2.2 Sep -Pak Vac 35cc. (silica C 18, 10g) 17 2.3 3.1 Solid Phase Extraction Steps Flow of Methodology 21 26 3.2 Molecular imprinting polymer technique 28 3.3 Solid Phase Extraction 30 4.1 Effect of

More

DNA purification and isolation of genomic DNA from ...

2014-6-23  5) Organic extraction using phenol (Wang and Rossman, 1994). Promega products like the Wizard® Plus SV Minipreps DNA Purification System and the PureYield™ Plasmid Systems combine the benefits of alkaline lysis with the rapid and easy purification by silica. This is done by using a silica-based membrane in a column format to bind the

More

DNA isolation methods - University of Alberta

2012-1-8  Organic extraction is a conventional technique that uses organic solvents to extract contaminants from cell lysates (3, 4). The cells are lysed using a detergent, and then mixed with phenol, chloroform, and isoamyl alcohol. The correct salt concentration and pH must be used during extraction to ensure that contaminants are separated into

More

[Study on analysis of trace phenol in small amount of ...

[Study on analysis of trace phenol in small amount of aqueous sample by liquid-liquid micro-extraction and gas chromatography]. [Article in Chinese] Zhang AL(1), Zhou JT, Han M, Teng LM, Huang LP, Wang D, Wang J. Author information: (1)School of Environmental Science and Engineering, Dalian University of Technology, Dalian 116012, China.

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SARS-CoV-2 RNA extraction using magnetic beads for

2020-7-8  nucleic acid extraction methods use either porous silica matrices embedded in a column (spin column) [12], a tip (TruTips) [13] or a suspension of microparticles. Microparticles can be separated from the lysate either by centrifugation or by a magnetic field if the microparticles' dense iron-containing cores are coated with porous silica [14].

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TLC ANALYSIS OF SOME PHENOLIC COMPOUNDS IN

2007-7-5  Samples for the TLC on silica gel were prepared using extraction with ether. 15 mL of sample and 15 mL of ether were mixed in separation funnel for the 5 min. After separa-tion, 15 mL of ether layer was vaporized up to 1 mL. Solutions of standard substances,

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(PDF) Paper prove Silica method better than Phenol ...

Forensic Science Highly Effective DNA Extraction Method for Nuclear Short Tandem Repeat Testing of Skeletal Remains from Mass Graves Jon Davoren1,2, Daniel Vanek1,3, Rijad Konjhodzić1,4, John Crews1,2, Edwin Huffine1,2, Thomas J. Parsons1 1 The International Commission Aim To quantitatively compare a silica extraction method with a on Missing Persons, Sarajevo, commonly used phenol

More

A Sample Extraction Method for Faster, More

2012-1-13  Three mechanistically different sample extraction methodologies, namely, silica spin columns, phenol-chloroform, and an automated magnetic capture of polymer-complexed DNA (via an Automate Express instrument), were compared for their abilities to purify nucleic acids from blood culture fluids for use in TaqMan assays for detection of Staphylococcus aureus. The extracts from silica

More

Photocatalytic degradation of phenol using silica SBA

2020-6-29  Silica SBA-16, an efficient photocatalyst support with cubic (bottle-ink) mesoporous structure, large specific surface area, large pore volume and uniform pore size and titanium oxide (TiO2), a semiconductor compound as the active site were synthesized facilely via one-pot method and photocatalysts denoted as x%TiO2/SBA-16 where x = 2, 5, 10 and 20, in order to examine their

More

Adsorption of phenol over bio-based silica/calcium ...

2020-10-13  A bio-based Silica/Calcium Carbonate (CS–SiO2/CaCO3) nanocomposite was synthesized in this study using waste eggshells (ES) and rice husks (RH). The adsorbents (ESCaCO3, RHSiO2 and, CS-SiO2/CaCO3) characterized using XRD show crystallinity associated with the calcite and quartz phase. The FTIR of ESCaCO3 shows the CO−23 group of CaCO3, while the spectra of RHSiO2

More

SELECTIVE SEPARATION OF PHENOLIC COMPOUND

2015-3-3  1.1 Molecular structure of phenol. 3 2.1 Molecular Imprinting Technique (MIP) 13 2.2 Sep -Pak Vac 35cc. (silica C 18, 10g) 17 2.3 3.1 Solid Phase Extraction Steps Flow of Methodology 21 26 3.2 Molecular imprinting polymer technique 28 3.3 Solid Phase Extraction 30 4.1 Effect of

More

DNA isolation methods - University of Alberta

2012-1-8  Organic extraction is a conventional technique that uses organic solvents to extract contaminants from cell lysates (3, 4). The cells are lysed using a detergent, and then mixed with phenol, chloroform, and isoamyl alcohol. The correct salt concentration and pH must be used during extraction to ensure that contaminants are separated into

More

SARS-CoV-2 RNA extraction using magnetic beads for

2020-7-8  nucleic acid extraction methods use either porous silica matrices embedded in a column (spin column) [12], a tip (TruTips) [13] or a suspension of microparticles. Microparticles can be separated from the lysate either by centrifugation or by a magnetic field if the microparticles' dense iron-containing cores are coated with porous silica [14].

More

TLC ANALYSIS OF SOME PHENOLIC COMPOUNDS IN

2007-7-5  Samples for the TLC on silica gel were prepared using extraction with ether. 15 mL of sample and 15 mL of ether were mixed in separation funnel for the 5 min. After separa-tion, 15 mL of ether layer was vaporized up to 1 mL. Solutions of standard substances,

More

Comparison of RNA isolation methods on RNA-Seq ...

2020-3-20  Differences in relative transcript abundance between phenol-extracted RNA and kit-extracted RNA. All of the RNA isolation methods yielded generally high quality RNA, as defined by a RIN of 9.0 or above, though the phenol extracted RNA averaged significantly higher RIN values than those isolated from the Direct-zol kit (9.96 vs. 9.33; P = 2 × 10 − 6, t-test) or the RNeasy kit (9.96 vs. 9.79 ...

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PRODUCTS Nucleic Acid Purification TOYOBO

Suitable for high throughput extraction of plasmid from E. coli cells. The extraction time is 10-15 minutes. Purified plasmid can be applied directly to sequencing, enzyme reaction, transformation, etc. Does not contain hazardous substances, such as phenol or chloroform.

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